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Add 5 ml of absolute ethanol or methanol to the 250 ml mark.Fill a cylinder (250 ml capacity) to the 245 ml mark with the saline solution.Caution: Glacial acetic acid is a corrosive chemical with an irritating vapor, therefore handle it with care in a well-ventilated room.Label the bottle, and store it at room temperature.Add the remaining acid solution, and mix well.Add about half of the acid solution to the acridine orange, and mix until the dye is completely dissolved.Fill a cylinder to the 98 ml mark with distilled water.Distilled water (D/W), acridine orange, and transfer to a.In bacterial vaginosis, the orange staining bacteria adhering to the green epithelial cells (clue cells) can be clearly seen.Īcetic acid, glacial (concentrated): 2 ml.The appearance of chromatoid bars of Entamoeba histolytica/ dispar in an acridine-stained smear is shown in this video clip as well as the above image.Trichomonas vaginalis : Orange-red with the yellow-green nucleus Results interpretation of Acridine orange
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Add a drop of saline or distilled water to the smear, and cover with a cover glass.Įxamine first with the 10X objective to see the distribution of fluorescing material, and then with the 40X objective to identify Trichomonas vaginalis and to detect yeast cells, bacteria, and parasites( chromatoid bars of Entamoeba histolytica/ dispar).Rinse the smear with physiological saline, and place the slide in a draining rack.Now, wash off the stain, and decolorize the smear with alcohol saline solution for 5–10 seconds.Cover the unfixed dried smear with the acridine orange acid stain for 5–10 seconds and smear fixes due to being fixative is contained in the stain.The procedure of Acridine orange staining Fluorescence microscope having a BG 12 exciter filter and No.Inoculating loop or sterile bamboo stick.It can also use to detect intracellular gonococci, meningococci, and other bacteria, particularly in blood cultures.Īcridine orange is a fluorochrome that causes DNA to fluoresce green and Acridine Orange Stain Introduction of Acridine orangeĪcridine orange is a fluorochrome stain and uses the rapid identification of Trichomonas vaginalis, yeast cells, and clue cells in vaginal smears. Stain in Microbiology chapter contains nearly all the stains those applicable in microbiology that contains name list of stains, introduction, uses, in some principle, procedure, result in interpretation, keynotes, etc.